Measuring 13C/15N chemical shift anisotropy in [13C,15N] uniformly enriched proteins using CSA amplification
نویسندگان
چکیده
منابع مشابه
NMR of enzymatically synthesized uniformly 13C15N-labeled DNA oligonucleotides.
A procedure for the enzymatic synthesis of uniformly 13C15N-labeled DNA oligonucleotides in milligram quantities for NMR studies is described. Deoxynucleotides obtained from microorganisms grown on 13C and 15N nutrient sources are enzymatically phosphorylated to dNTPs, and the dNTPs are incorporated into oligonucleotides using a 3'-5' exonuclease-deficient mutant of Klenow fragment of DNA polym...
متن کاملChemical shift anisotropy amplification.
A new NMR experiment which allows a measurement of the chemical shift anisotropy (CSA) tensor under magic angle spinning (MAS) is described. This correlates a fast MAS spectrum in the omega2 dimension with a sideband pattern in omega1 in which the intensities mimic those for a sample spinning at a fraction of the rate omega r/N, and these sidebands result from an amplification by a factor N of ...
متن کاملSolution NMR Measurement of Amide Proton Chemical Shift Anisotropy in 15N-Enriched Proteins. Correlation with Hydrogen Bond Length§
Cross-correlation between 15N-1H dipolar interactions and 1HN chemical shift anisotropy (CSA) gives rise to different relaxation rates of the doublet components of 1H-{15N} peptide backbone amides. Two schemes for quantitative measurement of this effect are described and demonstrated for samples of uniformly 15N-enriched ubiquitin and perdeuterated 15N-enriched HIV-1 protease. The degree of rel...
متن کاملSecondary structures of peptides and proteins via NMR chemical-shielding anisotropy (CSA) parameters.
Complete nuclear magnetic resonance (NMR) chemical-shielding tensors, sigma, have been computed at different levels of density-functional theory (DFT), within the gauge-including atomic orbital (GIAO) formalism, for the atoms of the peptide model For-L-Ala-NH2 as a function of the backbone dihedral angles phi and psi by employing a dense grid of 10 degrees. A complete set of rigorously orthogon...
متن کاملMR characterization of compartment shape anisotropy (CSA)
INTRODUCTION The orientational dependence of the diffusion-weighted MR signal intensity has been exploited to characterize the integrity and orientation of white-matter pathways in the brain. Anisotropy observed in traditional single pulsed field gradient (single-PFG) acquisitions is primarily a product of the interaction betweeen cellular membranes and diffusing water molecules suggesting the ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Solid State Nuclear Magnetic Resonance
سال: 2015
ISSN: 0926-2040
DOI: 10.1016/j.ssnmr.2015.09.002